Solutions for Tris/ glycine SDS-polyacrylamide gel electrophoresis

From Diseasepedia.net

Jump to: navigation, search
No8. Solutions for Tris/ glycine SDS-polyacrylamide gel electrophoresis
 6%  5ml  10ml  15ml  20ml  25ml  30ml  40ml  50ml
 H2O
 30% Acrylamide mix
 1.5M Tris (pH 8.8)
 10% SDS
 10% APS
 TEMED		  2.6
 1.0
 1.3
 0.05
 0.05
 0.004		  5.3
 2.0
 2.5
 0.1
 0.1
 0.008		  7.9
 3.0
 3.8
 0.15
 0.15
 0.012		  10.6
 4.0
 5.0
 0.2
 0.2
 0.016		  13.2
 5.0
 6.3
 0.25
 0.25
 0.02		  15.9
 6.0
 7.5
 0.3
 0.3
 0.024		  21.2
 8.0
 10.0
 0.4
 0.4
 0.032		  26.5
 10.0
 12.5
 0.5
 0.5
 0.04
 8%  5ml  10ml  15ml  20ml  25ml  30ml  40ml  50ml
 H2O
 30% Acrylamide mix
 1.5M Tris (pH 8.8)
 10% SDS
 10% APS
 TEMED		  2,3
 1.3
 1.3
 0.05
 0.05
 0.003		  4.6
 2.7
 2.5
 0.1
 0.1
 0.006		  6.9
 4.0
 3.8
 0.15
 0.15
 0.009		  9.3
 5.3
 5.0
 0.2
 0.2
 0.012		  11.6
 6.7
 6.3
 0.25
 0.25
 0.015		  13.9
 8.0
 7.5
 0.3
 0.3
 0.018		  18.5
 10.7
 10.0
 0.4
 0.4
 0.024		  23.2
 13.3
 12.5
 0.5
 0.5
 0.03
 10%  5ml  10ml  15ml  20ml  25ml  30ml  40ml  50ml
 H2O
 30% Acrylamide mix
 1.5M Tris (pH 8.8)
 10% SDS
 10% APS
 TEMED		  2.0
 1.7
 1.3
 0.05
 0.05
 0.002		  4.0
 3.3
 2.5
 0.1
 0.1
 0.006		  5.9
 5
 3.8
 0.15
 0.15
 0.006		  7.9
 6.7
 5.0
 0.2
 0.2
 0.008		  9.9
 8.3
 6.3
 0.25
 0.25
 0.01		  11.9
 10.0
 7.5
 0.3
 0.3
 0.012		  15.8
 13.3
 10.0
 0.4
 0.4
 0.016		  19.8
 16.7
 12.5
 0.5
 0.5
 0.02
 12%  5ml  10ml  15ml  20ml  25ml  30ml  40ml  50ml
 H2O
 30% Acrylamide mix
 1.5M Tris (pH 8.8)
 10% SDS
 10% APS
 TEMED		  1.6
 2.0
 1.3
 0.05
 0.05
 0.002		  3.3
 4.0
 2.5
 0.1
 0.1
 0.004		  4.9
 6.0
 3.8
 0.15
 0.15
 0.006		  6.6
 8.0
 5.0
 0.2
 0.2
 0.008		  8.2
 10.0
 6.3
 0.25
 0.25
 0.01		  9.9
 12.0
 7.5
 0.3
 0.3
 0.012		  13.2
 16.0
 10.0
 0.4
 0.4
 0.016		  16.5
 20.0
 12.5
 0.5
 0.5
 0.02
 15%  5ml  10ml  15ml  20ml  25ml  30ml  40ml  50ml
 H2O
 30% Acrylamide mix
 1.5M Tris (pH 8.8)
 10% SDS
 10% APS
 TEMED		  1.1
 2.5
 1.3
 0.05
 0.05
 0.002		  2.3
 5.0
 2.5
 0.1
 0.1
 0.004		  3.4
 7.5
 3.8
 0.15
 0.15
 0.006		  4.6
 10.0
 5.0 
 0.2
 0.2
 0.008		  5.7
 12.5
 6.3
 0.25
 0.25
 0.01		  6.9
 15.0
 7.5
 0.3
 0.3
 0.012		  9.2
 20.0
 10.0
 0.4
 0.4
 0.016		  11.4
 25.0
 12.5
 0.5
 0.5
 0.02
 STACK  1ml  2ml  3ml  4ml  5ml  6ml  8ml  10ml
 H2O
 30% Acrylamide mix
 1.0M Tris (pH 6.8)
 10% SDS
 10% APS
 TEMED		  0.68
 0.17
 0.13
 0.01
 0.01
 0.001		  1.4
 0.33
 0.25
 0.02
 0.02
 0.002		  2.1
 0.5
 0.38
 0.03
 0.03
 0.003		  2.7
 0.67
 0.5
 0.04
 0.04
 0.004		  3.4
 0.83
 0.63
 0.05
 0.05
 0.005  		 4.1
 1.0
 0.75
 0.06
 0.06
 0.006		  5.5
 1.3
 1.0
 0.08
 0.08
 0.008		  6.8
 1.7
 1.25
 0.1
 0.1
 0.01
Retrieved from "http://Diseasepedia.net/index.php/Solutions_for_Tris/_glycine_SDS-polyacrylamide_gel_electrophoresis"